CHEMISTRY OF THE VAN DEN BERGH REACTION
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چکیده
منابع مشابه
Chemistry of the Van Den Bergh Reaction
The form in which bilirubin exists in the blood is of considerable interest because of the observed differences in the van den Bergh reaction in disease and because of the relation of bilirubin to hemoglobin metabolism. All of the bilirubin in plasma reacts in 30 minutes with diazonium salts in acid 50 per cent methyl alcohol to give a red dye (1). A variable fraction of the total reacts with v...
متن کاملThe significance of the van den Bergh reaction.
Introduction A REASSESSMENT of the clinical significance of the van den Bergh reaction is rendered highly desirable by the development during the last 15 years of new methods for the determination of bilirubin in body fluids as well as for the estimation of the proportions of the alleged direct and indirect forms of bilirubin. Moreover, modern photo-electric devices permit an accurate measureme...
متن کاملThe standardization of the Van Den Bergh reaction for serum bilirubin.
N ATTEMPT TO STANDAJIDIZE a micromethod for the determination of serum bilirubin in premature infants, difficulty was encountered in the use of commercially available bilirubin preparations. Duplication of calibration curves previously reported in the literature could not be achieved, and data available on the van den Bergh reaction were not helpful in the solution of this problem. The persisti...
متن کاملThe separation of serum pigments giving the direct and indirect van den Bergh reaction.
Forty years ago van den Bergh developed a method for the estimation of bilirubin in serum by combining it in alcoholic solution with freshly diazotized sulphanilic acid. Shortly afterwards van den Bergh and Muller (1916) noted that by the omission of alcohol they could distinguish two types of reaction. Pigments from bile and serum from cases of obstructive jaundice developed a colour in the ab...
متن کاملFactors Affecting the Rate of Coupling of Bilirubin and Conjugated Bilirubin in the Van Den Bergh Reaction
Many workers have modified van den Bergh's method for determining bile pigment in serum (With, 1954). Two of the more satisfactory methods are those of Malloy and Evelyn (1937) and King and Coxon (1950). The present study was undertaken in order to explain differences in the results given by these two methods, and in an attempt to combine the desirable features of each, on a scale which was sui...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1940
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)73401-7